abstract：:Klebsiella oxytoca KMS005 (∆adhE∆ackA-pta∆ldhA) was metabolically engineered to improve 2,3-butanediol (BDO) yield. Elimination of alcohol dehydrogenase E (adhE), acetate kinase A-phosphotransacetylase (ackA-pta), and lactate dehydrogenase A (ldhA) enzymes allowed BDO production as a primary pathway for NADH re-oxidat...

journal_title：Metabolic engineering

authors： Jantama K,Polyiam P,Khunnonkwao P,Chan S,Sangproo M,Khor K,Jantama SS,Kanchanatawee S

更新日期：2015-07-01 00:00:00

abstract：:Improved supply of farnesyl diphosphate (FPP) is often considered as a typical strategy for engineering Saccharomyces cerevisiae towards efficient terpenoid production. However, in the engineered strains with enhanced precursor supply, the production of the target metabolite is often impeded by insufficient capacity o...

journal_title：Metabolic engineering

authors： Xie W,Lv X,Ye L,Zhou P,Yu H

更新日期：2015-07-01 00:00:00

abstract：:In this report, UbiE and UbiH in the quinone modification pathway (QMP) were identified in addition to UbiG as bottleneck enzymes in the CoQ10 biosynthesis by Rhodobacter sphaeroides. The CoQ10 content was enhanced after co-overexpression of UbiE and UbiG, however, accompanied by the accumulation of the intermediate 1...

journal_title：Metabolic engineering

authors： Lu W,Ye L,Lv X,Xie W,Gu J,Chen Z,Zhu Y,Li A,Yu H

更新日期：2015-05-01 00:00:00

abstract：:Cyclic guanosine monophosphate (cGMP) is a universal second messenger that is synthesized from guanosine triphosphate (GTP) by guanylyl cyclases (GCs) and hydrolyzed into guanosine monophosphate (GMP) by phosphodiesterases (PDEs). Small-molecule drugs that induce high cGMP levels in specialized tissues by boosting GC ...

journal_title：Metabolic engineering

authors： Kim T,Folcher M,Charpin-El Hamri G,Fussenegger M

更新日期：2015-05-01 00:00:00

abstract：:Lipogenic organisms provide an ideal platform for biodiesel and oleochemical production. Through our previous rational metabolic engineering efforts, lipogenesis titers in Yarrowia lipolytica were significantly enhanced. However, the resulting strain still suffered from decreased biomass generation rates. Here, we emp...

journal_title：Metabolic engineering

authors： Liu L,Pan A,Spofford C,Zhou N,Alper HS

更新日期：2015-05-01 00:00:00

abstract：:Cell metabolism is an important platform for sustainable biofuel, chemical and pharmaceutical production but its complexity presents a major challenge for scientists and engineers. Although in silico strains have been designed in the past with predicted performances near the theoretical maximum, real-world performance...

journal_title：Metabolic engineering

authors： Yang L,Srinivasan S,Mahadevan R,Cluett WR

更新日期：2015-03-01 00:00:00

abstract：:A novel strategy to finely control a large metabolic flux by using a "metabolic transistor" approach was established. In this approach a small change in the level or availability of an essential component for the process is controlled by adding a competitive reaction that affects a precursor or an intermediate in its ...

journal_title：Metabolic engineering

authors： Wu H,Tuli L,Bennett GN,San KY

更新日期：2015-03-01 00:00:00

abstract：:To obtain fast growing oil-rich microalgal strains has been urgently demanded for microalgal biofuel. Malic enzyme (ME), which is involved in pyruvate metabolism and carbon fixation, was first characterized in microalgae here. Overexpression of Phaeodactylum tricornutum ME (PtME) significantly enhanced the expression ...

journal_title：Metabolic engineering

authors： Xue J,Niu YF,Huang T,Yang WD,Liu JS,Li HY

更新日期：2015-01-01 00:00:00

abstract：:Single cell investigations have enabled unexpected discoveries, such as the existence of biological noise and phenotypic switching in infection, metabolism and treatment. Herein, we review methods that enable such single cell investigations specific to metabolism and bioenergetics. Firstly, we discuss how to isolate a...

journal_title：Metabolic engineering

authors： Vasdekis AE,Stephanopoulos G

更新日期：2015-01-01 00:00:00

abstract：:The promise of plants to serve as the green factories of the future is ever increasing. Plants have been used traditionally for construction, energy, food and feed. Bioactive compounds primarily derived from specialized plant metabolism continue to serve as important scaffold molecules for pharmaceutical drug producti...

journal_title：Metabolic engineering

authors： Yuan L,Grotewold E

更新日期：2015-01-01 00:00:00

abstract：:Metabolic pathways in cells must be sufficiently robust to tolerate fluctuations in expression levels and changes in environmental conditions. Perturbations in expression levels may lead to system failure due to the disappearance of a stable steady state. Increasing evidence has suggested that biological networks have...

journal_title：Metabolic engineering

authors： Lee Y,Lafontaine Rivera JG,Liao JC

更新日期：2014-09-01 00:00:00

abstract：:We observed that removing pantothenate (vitamin B5), a precursor to co-enzyme A, from the growth medium of Saccharomyces cerevisiae engineered to produce β-farnesene reduced the strain׳s farnesene flux by 70%, but increased its viability, growth rate and biomass yield. Conversely, the growth rate and biomass yield of ...

journal_title：Metabolic engineering

authors： Sandoval CM,Ayson M,Moss N,Lieu B,Jackson P,Gaucher SP,Horning T,Dahl RH,Denery JR,Abbott DA,Meadows AL

更新日期：2014-09-01 00:00:00

abstract：:3-Ketosteroid-△(1)-dehydrogenase (KstD) is a key enzyme involved in the microbial catabolism of sterols. Here, three homologues of KstD were characterized from Mycobacterium neoaurum ATCC 25795, showing distinct substrate preferences and transcriptional responses to steroids. Single deletion of any MN-kstD failed to r...

journal_title：Metabolic engineering

authors： Yao K,Xu LQ,Wang FQ,Wei DZ

更新日期：2014-07-01 00:00:00

abstract：:A typical characteristic of the butyric acid-producing Clostridium is coproduction of both butyric and acetic acids. Increasing the butyric acid selectivity important for economical butyric acid production has been rather difficult in clostridia due to their complex metabolic pathways. In this work, Clostridium acetob...

journal_title：Metabolic engineering

authors： Jang YS,Im JA,Choi SY,Lee JI,Lee SY

更新日期：2014-05-01 00:00:00

abstract：:Parthenolide, the main bioactive compound of the medicinal plant feverfew (Tanacetum parthenium), is a promising anti-cancer drug. However, the biosynthetic pathway of parthenolide has not been elucidated yet. Here we report on the isolation and characterization of all the genes from feverfew that are required for the...

journal_title：Metabolic engineering

authors： Liu Q,Manzano D,Tanić N,Pesic M,Bankovic J,Pateraki I,Ricard L,Ferrer A,de Vos R,van de Krol S,Bouwmeester H

更新日期：2014-05-01 00:00:00

abstract：:The modularity and versatility of an engineered functional reversal of the β-oxidation cycle make it a promising platform for the synthesis of longer-chain (C≥4) products. While the pathway has recently been exploited for the production of n-alcohols and carboxylic acids, fully capitalizing on its potential for the sy...

journal_title：Metabolic engineering

authors： Cintolesi A,Clomburg JM,Gonzalez R

更新日期：2014-05-01 00:00:00

abstract：:The biological nitrogen fixation carried out by some Bacteria and Archaea is one of the most attractive alternatives to synthetic nitrogen fertilizers. In this study we compared the effect of controlling the maximum activation state of the Azotobacter vinelandii glutamine synthase by a point mutation at the active sit...

journal_title：Metabolic engineering

authors： Ortiz-Marquez JC,Do Nascimento M,Curatti L

更新日期：2014-05-01 00:00:00

abstract：:The non-recyclable use of nitrogen fertilizers in microbial production of fuels and chemicals remains environmentally detrimental. Conversion of protein wastes into biofuels and ammonia by engineering nitrogen flux in Escherichia coli has been demonstrated as a method to reclaim reduced-nitrogen and curb its environme...

journal_title：Metabolic engineering

authors： Choi KY,Wernick DG,Tat CA,Liao JC

更新日期：2014-05-01 00:00:00

abstract：:3-Hydroxypropionic acid (3-HP) is an attractive platform chemical, which can be used to produce a variety of commodity chemicals, such as acrylic acid and acrylamide. For enabling a sustainable alternative to petrochemicals as the feedstock for these commercially important chemicals, fermentative production of 3-HP is...

journal_title：Metabolic engineering

authors： Chen Y,Bao J,Kim IK,Siewers V,Nielsen J

更新日期：2014-03-01 00:00:00

abstract：:Utilization of Candida glabrata overproducing pyruvate is a promising strategy for high-level acetoin production. Based on the known regulatory and metabolic information, acetaldehyde and thiamine were fed to identify the key nodes of carboligase activity reaction (CAR) pathway and provide a direction for engineering ...

journal_title：Metabolic engineering

authors： Li S,Xu N,Liu L,Chen J

更新日期：2014-03-01 00:00:00

abstract：:The intracellular redox state plays an important role in the cellular physiology that determines the efficiency of chemical and biofuel production by microbial cell factories. However, it is difficult to achieve optimal redox rebalancing of synthetic pathways owing to the sensitive responses of cellular physiology acc...

journal_title：Metabolic engineering

authors： Lim JH,Seo SW,Kim SY,Jung GY

更新日期：2013-11-01 00:00:00

abstract：:Control of gene and protein expression of both endogenous and heterologous genes is a key component of metabolic engineering. While a large amount of work has been published characterizing promoters for this purpose, less effort has been exerted to elucidate the role of terminators in yeast. In this study, we characte...

journal_title：Metabolic engineering

authors： Curran KA,Karim AS,Gupta A,Alper HS

更新日期：2013-09-01 00:00:00

abstract：:An iterative approach to optimising the accumulation of non-native long chain polyunsaturated fatty acids in transgenic plants was undertaken in Arabidopsis thaliana. The contribution of a number of different transgene enzyme activities was systematically determined, as was the contribution of endogenous fatty acid me...

journal_title：Metabolic engineering

authors： Ruiz-Lopez N,Haslam RP,Usher SL,Napier JA,Sayanova O

更新日期：2013-05-01 00:00:00

abstract：:Dietary carotenoids have been shown to be beneficial to health by decreasing the risk of many diseases. Attempts to enhance carotenoids in food crops have been successful although higher plants appear to resist big changes of carotenoid biosynthesis by metabolic engineering. Here we report the generation of a more nut...

journal_title：Metabolic engineering

authors： Huang JC,Zhong YJ,Liu J,Sandmann G,Chen F

更新日期：2013-05-01 00:00:00

abstract：:5-Aminovalerate (5AVA) is the precursor of valerolactam, a potential building block for producing nylon 5, and is a C5 platform chemical for synthesizing 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. Escherichia coli was metabolically engineered for the production of 5-aminovalerate (5AVA) and glutarate. When the...

journal_title：Metabolic engineering

authors： Park SJ,Kim EY,Noh W,Park HM,Oh YH,Lee SH,Song BK,Jegal J,Lee SY

更新日期：2013-03-01 00:00:00

abstract：:The rapK gene required for biosynthesis of the DHCHC starter acid that initiates rapamycin biosynthesis was deleted from strain BIOT-3410, a derivative of Streptomyces rapamycinicus which had been subjected to classical strain and process development and capable of robust rapamycin production at titres up to 250mg/L. ...

journal_title：Metabolic engineering

authors： Kendrew SG,Petkovic H,Gaisser S,Ready SJ,Gregory MA,Coates NJ,Nur-E-Alam M,Warneck T,Suthar D,Foster TA,McDonald L,Schlingman G,Koehn FE,Skotnicki JS,Carter GT,Moss SJ,Zhang MQ,Martin CJ,Sheridan RM,Wilkinson B

更新日期：2013-01-01 00:00:00

abstract：:Xylose, which is a major constituent of lignocellulosic biomass, was utilized for the production of poly(lactate-co-3-hydroxybutyrate) [P(LA-co-3HB)], having transparent and flexible properties. The recombinant Escherichia coli JW0885 (pflA(-)) expressing LA-polymerizing enzyme (LPE) and monomer supplying enzymes grow...

journal_title：Metabolic engineering

authors： Nduko JM,Matsumoto K,Ooi T,Taguchi S

更新日期：2013-01-01 00:00:00

abstract：:3-Hydroxypropionic acid (3-HP), an industrially important platform chemical, is used as a precursor during the production of many commercially important chemicals. Recently, recombinant strains of K. pneumoniae overexpressing an NAD(+)-dependent γ-glutamyl-γ-aminobutyraldehyde dehydrogenase (PuuC) enzyme of K. pneumon...

journal_title：Metabolic engineering

authors： Ashok S,Mohan Raj S,Ko Y,Sankaranarayanan M,Zhou S,Kumar V,Park S

更新日期：2013-01-01 00:00:00

abstract：:Successful redirection of endogenous resources into heterologous pathways is a central tenet in the creation of efficient microbial cell factories. This redirection, however, may come at a price of poor biomass accumulation, reduced cofactor regeneration and low recombinant enzyme expression. In this study, we propose...

journal_title：Metabolic engineering

authors： Solomon KV,Sanders TM,Prather KL

更新日期：2012-11-01 00:00:00

abstract：:Fumaric acid, a dicarboxylic acid used as a food acidulant and in manufacturing synthetic resins, can be produced from glucose in fermentation by Rhizopus oryzae. However, the fumaric acid yield is limited by the co-production of ethanol and other byproducts. To increase fumaric acid production, overexpressing endogen...

journal_title：Metabolic engineering

authors： Zhang B,Skory CD,Yang ST

更新日期：2012-09-01 00:00:00

abstract：:The glycolipid synthase MG517 from Mycoplasma genitalium catalyzes the glucosyl transfer from UDPGlc to diacylglycerol producing glycoglycerolipids (GGL) (Andrés et al., 2011). The enzyme was functional in E. coli accumulating GGL in the plasma membrane. A metabolic engineering strategy for GGL production was evaluate...

journal_title：Metabolic engineering

authors： Mora-Buyé N,Faijes M,Planas A

更新日期：2012-09-01 00:00:00

abstract：:Industrial production of semi-synthetic cephalosporins by Penicillium chrysogenum requires supplementation of the growth media with the side-chain precursor adipic acid. In glucose-limited chemostat cultures of P. chrysogenum, up to 88% of the consumed adipic acid was not recovered in cephalosporin-related products, b...

journal_title：Metabolic engineering

authors： Veiga T,Gombert AK,Landes N,Verhoeven MD,Kiel JA,Krikken AM,Nijland JG,Touw H,Luttik MA,van der Toorn JC,Driessen AJ,Bovenberg RA,van den Berg MA,van der Klei IJ,Pronk JT,Daran JM

更新日期：2012-07-01 00:00:00

abstract：:The biosynthesis of non-ribosomal peptides, many of which have pharmaceutical activities, is an evolutionary privilege of microorganisms. Capitalizing on the universal set of the Streptomyces lavendulae non-ribosomal peptide synthase BpsA and the Streptomyces verticillus 4'-phosphopantetheinyl transferase Svp, we have...

journal_title：Metabolic engineering

authors： Müller M,Ausländer S,Ausländer D,Kemmer C,Fussenegger M

更新日期：2012-07-01 00:00:00

abstract：:The field of Metabolic Engineering has recently undergone a transformation that has led to a rapid expansion of the chemical palate of cells. Now, it is conceivable to produce nearly any organic molecule of interest using a cellular host. Significant advances have been made in the production of biofuels, biopolymers a...

journal_title：Metabolic engineering

authors： Curran KA,Alper HS

更新日期：2012-07-01 00:00:00

abstract：:The construction of large DNA molecules that encode pathways, biological machinery, and entire genomes has been limited to the reproduction of natural sequences. However, now that robust methods for assembling hundreds of DNA fragments into constructs > 20 kb are readily available, optimization of large genetic elemen...

journal_title：Metabolic engineering

authors： Merryman C,Gibson DG

更新日期：2012-05-01 00:00:00

abstract：:As synthetic pathways built from exogenous enzymes become more complicated, the probability of encountering undesired interactions with host organisms increases, thereby lowering product titer. An emerging strategy to combat this problem is to spatially organize pathway enzymes into multi-protein complexes, where high...

journal_title：Metabolic engineering

authors： Lee H,DeLoache WC,Dueber JE

更新日期：2012-05-01 00:00:00

abstract：:This study focused on metabolic changes in the neuronal human cell line AGE1.HN upon increased ammonia stress. Batch cultivations of α(1)-antitrypsin (A1AT) producing AGE1.HN cells were carried out in media with initial ammonia concentrations ranging from 0mM to 5mM. Growth, A1AT production, metabolite dynamics and fi...

journal_title：Metabolic engineering

authors： Priesnitz C,Niklas J,Rose T,Sandig V,Heinzle E

更新日期：2012-03-01 00:00:00

abstract：:FK506, a widely used immunosuppressant, is produced by industrial fermentation processes using various Streptomyces species. Independently of the strain, structurally related compound FK520 is co-produced, resulting in complex and costly isolation procedures. In this paper, we report a chemobiosynthetic approach for e...

journal_title：Metabolic engineering

authors： Kosec G,Goranovič D,Mrak P,Fujs S,Kuščer E,Horvat J,Kopitar G,Petković H

更新日期：2012-01-01 00:00:00

abstract：:Optimizing the productivity of bioengineered strains requires balancing ATP generation and carbon atom conservation through fine-tuning cell respiration and metabolism. Traditional approaches manipulate cell respiration by altering air feeding, which are technically difficult especially in large bioreactors. An approa...

journal_title：Metabolic engineering

authors： Zhu J,Sánchez A,Bennett GN,San KY

更新日期：2011-11-01 00:00:00

abstract：:Cytochalasins are a group of fungal secondary metabolites with diverse structures and bioactivities, including cytochalasin E produced by Aspergillus clavatus, which is a potent anti-angiogenic agent. Here, we report the identification and characterization of the cytochalasin gene cluster from A. clavatus NRRL 1. As a...

journal_title：Metabolic engineering

authors： Qiao K,Chooi YH,Tang Y

更新日期：2011-11-01 00:00:00